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Infect. Immun. doi:10.1128/IAI.00532-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Immunization with Vibrio cholerae outer membrane vesicles induces protective immunity in mice

Howard Hughes Medical Institute and the Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, U.S.A.; Institut fuer Molekulare Biowissenschaften, Karl-Franzens-Universitaet Graz, Humboldtstrasse 50, 8010 Graz, Austria

^* To whom correspondence should be addressed. Email: andrew.camilli{at}tufts.edu.

	Abstract

The Gram-negative bacterium Vibrio cholerae releases outer membrane vesicles (OMVs) during growth. In this study we immunized female mice by the intranasal, intragastric or intraperitoneal routes with purified OMVs derived from V. cholerae. Independent of the route of immunization, mice induced a specific, high titer immune response of similar level against a variety of antigens present in the OMVs. After the last immunization, the half-maximum total immunoglobulin titer was stable over a three month period indicating that the immune response was long-lasting. The induction of specific isotypes was however dependent on the immunization route. IgA, for example, was only induced to a significant level by mucosal immunization, with the intranasal route generating the highest titers. We challenged the offspring of immunized female mice with V. cholerae via the oral route in two consecutive periods approximately 30 and 95 days after the last immunization. Regardless of the route of immunization, the offspring was protected against colonization with V. cholerae in both challenge periods. Our results show that both routes of mucosal immunization with OMVs derived from V. cholerae induce a long-term protective immune response against this gastrointestinal pathogen. These findings may contribute to the development of ‘non-living’, OMV-based vaccines against V. cholerae and other enteric pathogens, using a oral or intranasal routes of immunization.

This article has been cited by other articles:

• Schild, S., Nelson, E. J., Bishop, A. L., Camilli, A. (2009). Characterization of Vibrio cholerae Outer Membrane Vesicles as a Candidate Vaccine for Cholera. Infect. Immun. 77: 472-484 [Abstract] [Full Text]